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Once the run is full, retrieve the Shipping and delivery Cartridge with the instrument. The cell sample need to be at the bottom or side of the collection tube. Be aware: It is recommended to spin down the Shipping and delivery Cartridge inside a mini-centrifuge for just a couple seconds for comprehensive quantity Restoration.

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Cross-segment histology followed by hematoxylin and eosin (H&E) staining to assess the thickness of the smaller or massive airway epithelium

The readings can be carried out before the culture is evaluated for endpoint characterizations, like electrophysiology or airway marker expression.

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TEER measurements is often performed consistently, without having producing damage to the cell society. It is possible to conduct a weekly TEER time course to describe the barrier purpose during the whole process of ALI tradition differentiation.

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Notes: Use pipette suggestions to Carefully scratch The underside of each effectively in an effort 지방이식 to carry the monolayer off, then dissociate the cell clumps into a single-cell suspension by pipetting up and down 5 - 10 instances.

Take note: PFA fixation could be considered for assessing intracellular markers or performing analysis in a afterwards time.

Immediately thaw cells in the 37°C water bathtub by gently shaking the cryovial. Take out the vial when a small frozen cell pellet continues to be. Notice: It's important to operate speedily in the following techniques to make sure superior cell viability and recovery.